2-Aminopyridine is an important compound, which is used as intermediate for the synthesis of pharmaceuticalcompounds. The present work was aimed to assess the effect of Mr. Trivedi’s biofield energy treatment on the physical,thermal and spectral characteristics of 2-AP. The work was accomplished by dividing the sample in two parts i.e. one part wasremained untreated, and another part had received biofield energy treatment. Subsequently, the samples were analyzed usingvarious characterization techniques such as X-ray diffraction, differential scanning calorimetry, thermogravimetric analysis,ultra violet-visible spectroscopy, and Fourier transform infrared spectroscopy. The XRD analysis revealed a decrease incrystallite size of the treated sample (91.80 nm) as compared to the control sample (97.99 nm). Additionally, the result showedan increase in Bragg’s angle (2θ) of the treated sample as compared to the control. The DSC and Differential thermal analysisanalysis showed an increase in melting temperature of the treated 2-AP with respect to the control. Moreover, the latent heat offusion of the treated sample was increased by 3.08%. The TGA analysis showed an increase in onset of thermal degradation(Tonset), and maximum thermal decomposition temperature (Tmax) of the treated 2-AP as compared to the control sample.Additionally, the treated sample showed a reduction in weight loss as compared with the control indicating higher thermalstability of the sample. UV-visible analysis showed no changes in the absorption peak of the treated sample as compared to thecontrol. The FT-IR spectroscopic results showed downward shifting of C-H stretching vibration 2991→2955 cm-1 in treatedsample with respect to the control.